B-chromosomes are small piece of chromosomes that are present in excess of normal or requisite chromosomal complements. Several species in the genus Lilium are known to carry B chromosomes. Lilium amabile, a native Lilium species in Korea, showed complex chromosome complements including aneuploids and B chromosomes in their somatic cells. B-chromosome are often preferentially inherited and derived in many ways at pre-meiotic, meiotic, or post-meiotic divisions, but the molecular mechanisms were rarely available. We carried out a transcriptome sequencing and de novo assembly of L. amabile in plant being normal diploids (2n=24) and having B-chromosome plants (2n=24+1B). A total of 77,087 genes were assembled and 59,339 were expressed in both samples. The differentially expressed genes of B-chromosome plants versus control diploids (FDR 0.05) was 8,048, of which 4,157 were up-regulated and 3,891 were down-regulated in B-chromosome containing plants. Gene ontology and pathway enrichment analysis indicated that a majority of genes have similar functions. Comparative transcriptome analsis found 2,484 uniquely expressed genes in B-chromosome containing plants. Our transcriptome results enhance our understanding of the transcriptional regulations of L. amabile in response to having B-chromosome and provide a valuable resource for the future investigation.
B-chromosomes are small piece of chromosomes that are present in excess of normal or requisite chromosomal complements. Several species in the genus Lilium are known to carry B chromosomes. Lilium amabile, a native Lilium species in Korea, showed complex chromosome complements including aneuploids and B chromosomes in their somatic cells. B-chromosome are often preferentially inherited and derived in many ways at pre-meiotic, meiotic, or post-meiotic divisions, but the molecular mechanisms were rarely available. We carried out a transcriptome sequencing and de novo assembly of L. amabile in plant being normal diploids (2n=24) and having B-chromosome plants (2n=24+1B). A total of 77,087 genes were assembled and 59,339 were expressed in both samples. The differentially expressed genes of B-chromosome plants versus control diploids (FDR 0.05) was 8,048, of which 4,157 were up-regulated and 3,891 were down-regulated in B-chromosome containing plants. Gene ontology and pathway enrichment analysis indicated that a majority of genes have similar functions. Comparative transcriptome analsis found 2,484 uniquely expressed genes in B-chromosome containing plants. Our transcriptome results enhance our understanding of the transcriptional regulations of L. amabile in response to having B-chromosome and provide a valuable resource for the future investigation.
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