The genus Onobrychis is a member of the Fabaceae family with approximatelly 170 perennial and annual species. Some species of the genus are important fodder crops. Previous cytogenetic studies in genus Onobrychis are limited to chromosome counts and construction of karyotypes based on classical staining methods. New molecular cytogenetics techniques have not yet been applied to investigate Onobrychis genomes. The objective of this study was to explore usage of flow cytometer and in situ hybridisation (FISH) methods on Onobrychis species for the first time. The present study reports genome size of 200 Onobrychis accessions belong to approximatelly 25 different species and distribution patterns of rDNAs in some of the species. Based on the results of the study, mean 2C DNA content of the Onobrychis species varied between 2.73 pg and 0.70 pg as the differences among species were statistically significant. Species generated two clearly distinquishable groups according to mean 2C DNA content. Two different essential chromosome number such as n=7 and n=8 were counted in the study as the majority of the accessions were polyploid with 2n=28 chromosomes. Based on the results of the FISH analysis, diploid species had distinctive distribution patterns of rDNA loci, whereas polyploids analysed in the study revealed similar rDNA organisation.
Acknowledgements: This study was supported by a TUBITAK grant (TOVAG-215O526).
The genus Onobrychis is a member of the Fabaceae family with approximatelly 170 perennial and annual species. Some species of the genus are important fodder crops. Previous cytogenetic studies in genus Onobrychis are limited to chromosome counts and construction of karyotypes based on classical staining methods. New molecular cytogenetics techniques have not yet been applied to investigate Onobrychis genomes. The objective of this study was to explore usage of flow cytometer and in situ hybridisation (FISH) methods on Onobrychis species for the first time. The present study reports genome size of 200 Onobrychis accessions belong to approximatelly 25 different species and distribution patterns of rDNAs in some of the species. Based on the results of the study, mean 2C DNA content of the Onobrychis species varied between 2.73 pg and 0.70 pg as the differences among species were statistically significant. Species generated two clearly distinquishable groups according to mean 2C DNA content. Two different essential chromosome number such as n=7 and n=8 were counted in the study as the majority of the accessions were polyploid with 2n=28 chromosomes. Based on the results of the FISH analysis, diploid species had distinctive distribution patterns of rDNA loci, whereas polyploids analysed in the study revealed similar rDNA organisation.
Acknowledgements: This study was supported by a TUBITAK grant (TOVAG-215O526).
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