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CmSat162 and CmSat189, new satellite DNAs in Cucumis melo L.

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Melon karyotyping still remains a challenge, due to not only the small chromosome sizes and poor stainability but also the limitation of cytological markers which commonly rely on ribosomal RNA genes (45S rDNA and 5S rDNA) and centromere repeat as the probes. Satellite DNAs (SatDNAs), occupying significant portion in Cucurbitaceae within their genomes, are making them valuable markers for chromosome identification. By utilizing melon genome database, we identified and found new SatDNAs in melon, namely CmSat162 and CmSat189, which were successfully hybridized to melon somatic cells, pachytene chromosomes and extended DNA fibers. CmSat162, a new centromeric SatDNA, was hybridized into 12 pairs of melon chromosomes and co-localized with Cmcent probe. In addition, CmSat189, located on specific region of each chromosome, allowed the discrimination and characterization of individual homologous chromosomes in melon. We also demonstrate that the transcription of these SatDNAs may have important function for maintaining centromere structures in melon. Our results suggest that CmSat162 and CmSat189 are useful for melon karyotyping. Additionally, those probes may contribute to integrate the physical, chromosomal and genetic maps for genome sequencing project.

Jul 05, 2018 02:15 PM - 02:30 PM(UTC)
Venue : 2B7 - Building 2
20180705T1415 20180705T1430 UTC CmSat162 and CmSat189, new satellite DNAs in Cucumis melo L.

Melon karyotyping still remains a challenge, due to not only the small chromosome sizes and poor stainability but also the limitation of cytological markers which commonly rely on ribosomal RNA genes (45S rDNA and 5S rDNA) and centromere repeat as the probes. Satellite DNAs (SatDNAs), occupying significant portion in Cucurbitaceae within their genomes, are making them valuable markers for chromosome identification. By utilizing melon genome database, we identified and found new SatDNAs in melon, namely CmSat162 and CmSat189, which were successfully hybridized to melon somatic cells, pachytene chromosomes and extended DNA fibers. CmSat162, a new centromeric SatDNA, was hybridized into 12 pairs of melon chromosomes and co-localized with Cmcent probe. In addition, CmSat189, located on specific region of each chromosome, allowed the discrimination and characterization of individual homologous chromosomes in melon. We also demonstrate that the transcription of these SatDNAs may have important function for maintaining centromere structures in melon. Our results suggest that CmSat162 and CmSat189 are useful for melon karyotyping. Additionally, those probes may contribute to integrate the physical, chromosomal and genetic maps for genome sequencing project.

2B7 - Building 2 GSA2018_APCC6 GSACC62018@canberra.edu.au
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