The potential use of human stem cell lines for therapy makes it essential to ensure that they have a normal genome. They often don’t.
Recurrent numerical and structural chromosome imbalances are acquired by pluripotent stem cells (hPSCs) during culture. Full and partial trisomies of chromosomes 8, 12, 17, 20 and X are common. Structural changes resulting in gains in critical regions of the long arm of chromosome 1 and chromosome 20 are not unusual. Abnormalities are similar between human embryonic stem cells and induced pluripotent stem cells. The changes are also consistent among varied derivation protocols, culture conditions, and geographic locations. Questions arise as to the cause and effect of this phenomenon. The tendency of hPSCs to become cytogenetically abnormal may have substantial impact on their use in regenerative medicine.
The potential use of human stem cell lines for therapy makes it essential to ensure that they have a normal genome. They often don’t.
Recurrent numerical and structural chromosome imbalances are acquired by pluripotent stem cells (hPSCs) during culture. Full and partial trisomies of chromosomes 8, 12, 17, 20 and X are common. Structural changes resulting in gains in critical regions of the long arm of chromosome 1 and chromosome 20 are not unusual. Abnormalities are similar between human embryonic stem cells and induced pluripotent stem cells. The changes are also consistent among varied derivation protocols, culture conditions, and geographic locations. Questions arise as to the cause and effect of this phenomenon. The tendency of hPSCs to become cytogenetically abnormal may have substantial impact on their use in regenerative medicine.
2B7 - Building 2 GSA2018_APCC6 GSACC62018@canberra.edu.au
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