The use of DNA derived from environmental samples, commonly referred to as environmental DNA or eDNA, is a powerful tool for monitoring the distribution of species. Environmental DNA based monitoring can use either a target approach, through the use of species-specific primers, or entire species communities can be characterized through the amplification of barcoding regions with universal primers and High-Throughput Sequencing of amplicon libraries (i.e. eDNA metabarcoding). While both approaches have been used extensively to monitor the distribution of aquatic species, a formal comparison of their performance is lacking. We will present the results of an extensive eDNA-based monitoring survey conducted in the Blakney Creek catchment (NSW). This catchment contains a population of the endangered Southern pygmy perch (SPP) but the long-term persistence of this population is threatened by the presence and continued spread of two invasive fish species, redfin perch (Perca fluviatilis) and common carp (Cyprinus carpio). Both targeted eDNA monitoring and eDNA metabarcoding was used to determine the distribution of the invasive redfin perch within the system and to evaluate the detection sensitivity of both monitoring approaches. Furthermore, seasonal sampling data was obtained to determine the influence of seasonality on both eDNA-based survey methods.
The use of DNA derived from environmental samples, commonly referred to as environmental DNA or eDNA, is a powerful tool for monitoring the distribution of species. Environmental DNA based monitoring can use either a target approach, through the use of species-specific primers, or entire species communities can be characterized through the amplification of barcoding regions with universal primers and High-Throughput Sequencing of amplicon libraries (i.e. eDNA metabarcoding). While both approaches have been used extensively to monitor the distribution of aquatic species, a formal comparison of their performance is lacking. We will present the results of an extensive eDNA-based monitoring survey conducted in the Blakney Creek catchment (NSW). This catchment contains a population of the endangered Southern pygmy perch (SPP) but the long-term persistence of this population is threatened by the presence and continued spread of two invasive fish species, redfin perch (Perca fluviatilis) and common carp (Cyprinus carpio). Both targeted eDNA monitoring and eDNA metabarcoding was used to determine the distribution of the invasive redfin perch within the system and to evaluate the detection sensitivity of both monitoring approaches. Furthermore, seasonal sampling data was obtained to determine the influence of seasonality on both eDNA-based survey methods.
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